Aggregation induced emission aie is an abnormal phenomenon that is observed with certain organic luminophores fluorescent dyes. Aggregationinduced emission for highly selective and sensitive fluorescent biosensing and cell imaging liang huang, liming dai department of macromolecular science and engineering, center of advanced science and engineering for carbon case4carbon, case western reserve university, 10900 euclid avenue, cleveland, ohio 44106. Photoactivatable probes for lipid droplets ldsspecific livecell imaging are powerful tools for investigating their biological functions through precise spatial and. Us10264976b2 biocompatible flavonoid compounds for. A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging bin li, a tianhong chen, b zhipeng wang, d zhiming guo, d jhair pena, a lintao zeng bc and jinfeng xing a. Inflammation is a protective response to stimuli trauma, which can also lead to severe tissue injury. Gelation process visualized by aggregationinduced emission. A cyanosubstituted diarylethlene derivative aggregationinduced emission aie dye with two amino endgroups and 4,4.
Facile fabrication of aggregationinduced emission based. Sep 12, 20 edited by professor tang, who first discovered this phenomenon, this 2volume reference addresses the fundamentals of aggregationinduced emission aie. On the one hand, novel bioprobes with superior physicochemical. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the. Most organic compounds have planar structures and higher photoemission efficiencies in solution than in the solid state. Since the light emission was induced by aggregate formation, professor tang termed the process aggregationinduced emission aie. Methods in molecular biology methods and protocols, vol 991. Due to the great importance of interface, it is necessary to visualize its structures and monitor the corresponding dynamic processes. Multiplexed imaging detection of live cell intracellular. Fullrange intracellular ph sensing by an aggregation. In addition, a dna aptamer specific to nucleolin was functionalized on the surface of aiefsnps for targeted cell imaging. The shift in the fluorescence from green to blue after interacting with the serum proteins in the cell culture conditions was also observed.
Aggregationinduced emissionactive rutheniumii complex of. Photoactivatable aggregationinduced emission probes for lipid. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic sensing, biomolecule detection, and cell imaging. Aggregationinduced emissionbased fluorescence probe for fast. In the current study, we induced apoptosis in hela cells with h 2 o 2 and used nuclear dye hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregationinduced emission. Aie is a novel photophysical phenomenon, which offers a new platform for researchers to look into the lightemitting processes from luminogen aggregates, from which useful information on structureproperty. Aggregationinduced emission of tetraarylethene luminogens. Pdf a novel aggregationinduced emission fluorescent. Flavonoid compounds may be prepared that are selective for certain cell organelles, and may be used as biological imaging agents. Fluorescent microscopy and flow cytometry studies both revealed highly selective fluorescence staining of mcf7 a cancer cell line with nucleolin overexpression over mcf10a normal cells by the aptamerfunctionalized. In this work, we have successfully developed ultrabright organic dots with. Restriction of intramolecular motion is the working mechanism of aggregation. Pdf aggregationinduced emission aie dyes have received.
A facile and simple onestep method was developed to fabricate aptamerdecorated selfassembled organic dots with aggregationinduced emission aie characteristics. Aggregation induced emission aie is the fluorogenic emission behavior in which the fluorescence is significantly enhanced due to the restriction of intramolecular motion when the fluorogens. Authors contributing to rsc publications journal articles, books or book. Aggregationinduced emission aie is a novel photophysical phenomenon which offers a new platform applications for researchers to look into the lightemitting processes from luminogen aggregates, from which useful information on structureproperty relationships may be collected and mechanistic insights may be gained.
Cell imaging cells were grown in a 35 mm petri dish with a coverslip at 37 c. Aggregationinduced emission enhancement and cell imaging of a novel carbazolnyltriphenylaminebodipy q. Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregationinduced emission aie properties, which can be used for longterm cell tracking. A fluorescent bioprobe comprising one or more fluorogens labeled on biomolecules. Applications is the first book to explore the high tech. Aggregation induced emission of fluorescent bioprobes and methods of using. A novel aggregationinduced emission fluorescent probe for nucleic acid detection and its applications in cell imaging author links open overlay panel xiaowei xu shengyong yan yimin zhou rong huang yuqi chen jiaqi wang xiaocheng weng xiang zhou. Preparation of aggregationinduced emission dots for longterm twophoton cell imaging article pdf available march 2015 with 141 reads how we measure reads. Compared with conventional fluorophores which are often quenched in the aggregate state or at high concentration due to concentration quenching or aggregation caused quenching, tetraphenylethene tpebased organic fluorogens exhibit an extraordinary aggregation induced emission aie feature, providing a ne. Preparation and selfassembly of amphiphilic polymer with. Seeing stem cells at work in vivo pubmed central pmc. May 22, 2014 a cyanosubstituted diarylethlene derivative aggregationinduced emission aie dye with two amino endgroups and 4,4.
Aggregation induced emission active cytophilic fluorescent bioprobes for longterm cell tracking. A phresponsive drug delivery system with an aggregationinduced emission feature for cell imaging and intracellular drug delivery. Aggregationinduced emission aie of complexes cu1cu4 in pbsdmso 99. Pdf preparation of aggregationinduced emission dots for. The concept of aggregationinduced emission aie changes this general belief and provides a new stage for. Easily accessible aggregationinduced emission probe for. In this study, fsnps hybridized with aggregation induced emission aie luminogens namely fsnpsd were successfully fabricated by a surfactantfree solgel method. Fabrication of fluorescent silica nanoparticles with. Fluorescent silica nanoparticles fsnps can provide highintensity and photostable fluorescent signals as a probe for biomedical analysis. The fluorescence images were taken under confocal microscope through irradiation at 514 nm with 7% laser power. Imaging intracellular anticancer drug delivery by self. Addition of poor solvents induces the molecules to aggregate, which turns the emission on and boosts their luminescence efficiencies dramatically aggregationinduced emission or aie. Xiaofan ji, yang li, hu wang, run zhao, guping tang, feihe huang. Tem images were obtained on a jem2100f field emission transmission electron microscope operated at an acceleration voltage.
Aggregationinduced emissionactive rutheniumii complex. Photostable fluorescent organic dots with aggregationinduced. Photostable fluorescent organic dots with aggregation. We utilize the objectivebased total internal reection microscopy combined with the living cell incubation. Aggregationinduced emission aie molecules eliminate the. Aggregationinduced emission luminogenembedded silica. Supramolecular hyaluronic assembly with aggregation. Synthesis, solvatochromism, aggregationinduced emission and cell imaging of tetraphenylethenecontaining bodipy derivatives with large stokes shifts. Therefore, these aiebased compounds can be used as selective and cell compatible fluroescent bioprobes for longterm live cell tracking and imaging. Aggregationinduced emission aie is a novel photophysical phenomenon which offers a new platform applications for researchers to look into the lightemitting processes from luminogen. Addition of poor solvents induces the molecules to aggregate, which turns the emission on and boosts their luminescence efficiencies dramatically aggregation induced emission or aie. Ultrafast delivery of aggregationinduced emission nanoparticles and pure organic phosphorescent nanocrystals by saponin encapsulation alexander nicol. Aggregationinduced emission enhancement and cell imaging. While in 2001, we discovered a novel phenomenon of aggregationinduced emission.
In this study, fsnps hybridized with aggregationinduced. The book presents an overview of this rapidly emerging and exciting area of research, inviting scientists to renew their photophysical knowledge and stimulate new developments in the field. Herein, we develop a novel strategy for designing a fluorescence probe. In vitro cytotoxicity of fluorescent silica nanoparticles. Cell imaging experiments were also carried out by incubation with hepg2. A fluorescent sensor based on aggregationinduced emission. Photobleaching resistance of this synthesized nanoparticle is tested and compared with its similar counterpart, which proves its superiority and capability of. Otherwise said, these fluorophores or fluorescent dyes are much more emissive in solution. Moreover, the acq dyes with highly planar conjugated structure also exhibit small stokes shift and suffer from reabsorption of the dye. Recent progress of bodipy dyes with aggregationinduced emission. Live cell studies reveal that aiefa is capable of imaging endogenous and exogenous fa in living cells. Aggregationinduced emission by tang, ben zhong ebook. Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregation induced emission aie properties, which can be used for longterm cell tracking.
A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging bin li, a tianhong chen, b zhipeng wang, d zhiming guo. An amphiphilic polymer bearing tetraphenylethene tpe moiety was synthesized by convenient reactions. Aggregationinduced emission for highly selective and sensitive fluorescent biosensing and cell imaging liang huang, liming dai department of macromolecular science and engineering, center. It has been a textbook knowledge that chromophore aggregation generally quenches light emission. These obtained rohfda fons were characterized by a series of. In addition, our recent discovery that aiegens with high brightness and efficient reactive oxygen species generation in the aggregate state further expand their applications in imageguided cancer surgery and therapy. Alternative cell tracking methods are therefore desired to attain reliable, stable, and efficient labeling over a long time. Aggregation induced emission tells us molecular aggregation. The polymer exhibits unique aggregationinduced emission aie characteristics and. Recently developed organic farrednearinfrared dots with aggregationinduced emission have also been demonstrated to be useful as longterm noninvasive cell tracers. Construction of metal complexbased aieactive materials with metaltoligand charge transfer mlct, ligandtometal charge transfer lmct emission together with the ligandcentered and intraligand lcilct emission is a challenging task. Photobleaching resistance of this synthesized nanoparticle is tested and compared with its similar counterpart, which proves its superiority and capability of long term uorescence emission.
Aggregationinduced emission by ben zhong tang overdrive. Aggregationinduced emission aie is an abnormal phenomenon that is observed with certain organic luminophores fluorescent dyes most organic compounds have planar structures and higher. Recent progress of bodipy dyes with aggregationinduced. The existing antiinflammatory drugs, such as corticosteroids and glucocorticoids, generally exhibit side effects and poor accumulation in inflammatory tissue. The discovery of the aie effect opens a new avenue for the development.
A fluorescent sensor based on aggregationinduced emission dois. Aie is a novel photophysical phenomenon, which offers a. A novel aggregationinduced emission fluorescent probe for nucleic acid detection and its applications in cell imaging. Aggregationinduced emissionfluorophores and applications. The development of red emissive aggregation induced emission aie active probes for organellespecific imaging is of great importance. The photoluminescence pl of t 2 tps and tpe layers adsorbed on the tlc plates can be turned off and on continuously and reversibly by solvent exposure.
Oct 04, 2017 the aggregation induced emission aie is the phenomena that the fluorescence intensity is low in solution, the aggregated state shows strong emission. Accompanied by other pseudo in situ investigations, we propose. Porphyrinbased nanomaterials and their applications for. Dual aggregation induced emission for enhanced fluorescence sensing of furin activity in vitro and in living cells. Fullrange intracellular ph sensing by an aggregationinduced emissionactive twochannel ratiometric fluorogen sijie chen, yuning hong, yang liu, jianzhao liu, chris w. Ultrabright organic dots with aggregationinduced emission. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the areas of. Conversion of the fluorescent properties depending on the aggregated states can also provide information on the evolution of the aggregation processes. Fabrication of aggregationinduced emission based fluorescent. After incubation with indtpa 5 m for 15 min, the cells were washed with pbs for three times. Aggregationinduced emission aieactive fluorescent probes with. Fluorescent dyes with aggregationinduced emission provide. Recent years have witnessed the fast growth of fluorogens with aggregationinduced emission characteristics aiegens in biomedical research.
Hence, a theranostic nanoplatform with serial reactive oxygen species ros responsiveness and twophoton aie bioimaging has been. A novel aggregationinduced emission fluorescent probe for. Edited by professor tang, who first discovered this phenomenon, this 2volume reference addresses the fundamentals of aggregationinduced emission aie. Oct 25, 2019 synthesis, solvatochromism, aggregationinduced emission and cell imaging of tetraphenylethenecontaining bodipy derivatives with large stokes shifts.
Jan 28, 20 photostable fluorescent organic dots with aggregation induced emission aie dots for noninvasive longterm cell tracing. Novel tetraphenylethylene diol amphiphile with aggregation. Jun 23, 2016 here we show the entire gelation process of chitosan liohurea aqueous system by aggregation induced emission fluorescent imaging. Aggregationinduced emission aieactive compounds are attractive. Xiaomei liu and gaolin liang cas key laboratory of soft matter chemistry, department of chemistry, university of science and technology of china, hefei, anhui 230026, china hefei corresponding author. Aggregationinduced emissionbased fluorescence probe for fast and. Visualizing and monitoring interface structures and. A novel crosslinked nanoparticle with aggregationinduced. In this paper, we report a set of novel aieactive fluorescent probes containing pyridiniums.
Facile fabrication of aggregationinduced emission based red. Moreover, the acq dyes with highly planar conjugated structure also exhibit small stokes shift and suffer from reabsorption of the dye emission, further reducing their emission efficiency 8. It is common knowledge that chromophore aggregation usually quenches light emission. The aggregationinduced emission aie is the phenomena that the fluorescence intensity is low in solution, the aggregated state shows strong emission. Aggregationinduced emission enhancement and living cell. Similaritybetweenaieandjaggregate emission is in suppression on aggregation of mobility in molecular segments that. The emergence of the aggregationinduced emission aie concept significantly. The development of red emissive aggregationinduced emission aie active probes for organellespecific imaging is of great importance. Dual aggregationinduced emission for enhanced fluorescence. Organelles that may be imaged with flavonoid compounds include. Us9409928b2 aggregation induced emission active cytophilic. The fsnpsd were spherical, monodisperse and uniform in size, with an average diameter of. Similaritybetweenaieandjaggregate emission is in suppression on aggregation of mobility in molecular segments that normally results in quenching.
Supplementary movie avi 1011k supplementary information pdf 1175k. Fullrange intracellular ph sensing by an aggregationinduced. A phresponsive drug delivery system with an aggregation induced emission feature for cell imaging and intracellular drug delivery. Aggregationinduced emission enhancement and living cell imaging of novel diarylanthracene conjugated dyes c. This is the first time that erythrocytes have been visualized in the brain through a fluorescence. Herein, supramolecular assembly with aie effect was constructed in two stages, where adamantane modified tetraphenylethene selfassembly emitted weak fluorescence, and then after adding. The concept of aggregation induced emission aie changes this general belief and provides a new stage for the exploration of practical luminescent materials.